Takano-Yamamoto T, Takemura T, Kitamura Y, Nomura S
Department of Orthodontics, Faculty of Dentistry, Osaka University, Japan.
J Histochem Cytochem. 1994 Jul;42(7):885-96. doi: 10.1177/42.7.8014472.
We investigated the gene expression for non-collagenous proteins in periodontal ligament (PDL) by in situ hybridization histochemistry with a non-radioisotopic probe with cRNAs for osteocalcin (Osc), osteonectin (Osn), and osteopontin (Opn) in rat maxillary dento-alveolar unit containing molars and intact PDL. A highly intense positive signal for Osn and Osc mRNAs was expressed at all distal surfaces of the interradicular septum of buccal roots of the upper second molar in 7-week-old Sprague-Dawley male rats. Cells showing positive signals for Osn and Osc mRNAs were osteoblasts and osteoprogenitor cells. The distribution of Opn mRNA-positive signal was demonstrable at the mesial surface of the interradicular septum of buccal roots, where physiological bone resorption was specifically restricted during physiological tooth movement. Opn mRNA was expressed in cells on the bone resorption surface, including osteoclasts, and osteocytes. A moderately intense positive signal for Osn mRNA was distributed in fibroblasts throughout the ligament. Odontoblasts and pre-mature odontoblasts exhibited a strong signal for Osn and Osc mRNA. Cementoblasts and cementocytes were positive for Osn, Osc, and Opn mRNAs. These findings suggest physiological roles of Osc, Osn, and Opn in bone remodeling, PDL remodeling, dentinogenesis, and cementogenesis.
我们采用非放射性cRNA探针原位杂交组织化学方法，研究了大鼠上颌含磨牙和完整牙周韧带的牙-牙槽单元中牙周韧带（PDL）非胶原蛋白的基因表达，这些探针分别针对骨钙素（Osc）、骨连接蛋白（Osn）和骨桥蛋白（Opn）。在7周龄雄性Sprague-Dawley大鼠上颌第二磨牙颊根根间中隔的所有远中面，均表达出Osn和Osc mRNA的高强度阳性信号。显示Osn和Osc mRNA阳性信号的细胞为成骨细胞和骨祖细胞。Opn mRNA阳性信号分布于颊根根间中隔的近中面，在此处，生理性牙齿移动过程中生理性骨吸收受到特异性限制。Opn mRNA在包括破骨细胞和骨细胞在内的骨吸收表面的细胞中表达。Osn mRNA的中等强度阳性信号分布于整个韧带的成纤维细胞中。成牙本质细胞和前成牙本质细胞对Osn和Osc mRNA显示强信号。成牙骨质细胞和牙骨质细胞对Osn、Osc和Opn mRNA呈阳性反应。这些发现提示Osc、Osn和Opn在骨重塑、牙周韧带重塑、牙本质形成和牙骨质形成中具有生理作用。
