Crott L S, Valim Y M, Silva C L, Barbosa J E
Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Brazil.
J Med Vet Mycol. 1993;31(1):17-27.
We investigated the capacity of an alkali-insoluble cell wall polysaccharide fraction (F1) of Paracoccidioides brasiliensis to induce rat polymorphonuclear neutrophil (PMN) migratory and chemiluminescence (CL) responses. Normal rat serum pre-incubated with F1 induced a chemotactic neutrophil response which was fully abolished by heat-inactivation. The participation of the alternative complement pathway was more effective than that of the classical pathway since depletion of factor B by heating at 50 degrees C reduced PMN migration, whereas blockade of the classical pathway with EGTA left the migratory response practically unchanged. Opsonized serum F1 induced a significant release of oxygen radicals from PMN as measured by CL. The complement system was also found to be involved in this activity since serum inactivation at 56 degrees C altered the CL response. In addition to complement-derived fragments, other serum opsonins, probably cross-reacting antibodies, were required for optimal interaction between PMN and opsonized particles. These results contribute to the understanding of the role of fungal components and of the complement system in the inflammatory response observed in paracoccidioidomycosis.
我们研究了巴西副球孢子菌的一种碱不溶性细胞壁多糖组分（F1）诱导大鼠多形核中性粒细胞（PMN）迁移和化学发光（CL）反应的能力。与F1预孵育的正常大鼠血清诱导了趋化性中性粒细胞反应，该反应在热灭活后完全消失。替代补体途径的参与比经典途径更有效，因为在50℃加热使B因子耗竭会降低PMN迁移，而用乙二醇双四乙酸（EGTA）阻断经典途径对迁移反应几乎没有影响。调理素化血清F1通过CL检测诱导PMN显著释放氧自由基。还发现补体系统参与了该活性，因为在56℃使血清失活会改变CL反应。除了补体衍生片段外，其他血清调理素（可能是交叉反应抗体）对于PMN与调理素化颗粒之间的最佳相互作用也是必需的。这些结果有助于理解真菌成分和补体系统在副球孢子菌病中观察到的炎症反应中的作用。
