Munk M E, Da Silva W D
Departamento de Imunologia, Universidade de São Paulo, Brazil.
J Med Vet Mycol. 1992;30(6):481-4.
The yeast form of Paracoccidioides brasiliensis strain Pb18 was able to activate C3 of normal human serum diluted in phosphate-buffered saline or EGTA-MgCl2 in vitro. C3 convertase was also permissive when Pb18 cells were pre-treated with a pool of immune serum from patients with paracoccidioidomycosis and incubated in serum diluted in EDTA-CaCl2. The components C3, and fragments C3c, C3d, C3g, factor H, factor B, C4 and C5b-9 were demonstrated on the Pb18 cell surface by immunofluorescence although no effect was seen on fungal viability.
巴西副球孢子菌Pb18菌株的酵母形式能够在体外激活稀释于磷酸盐缓冲盐水或EGTA - MgCl₂中的正常人血清的C3。当用来自副球孢子菌病患者的免疫血清池预处理Pb18细胞，并在稀释于EDTA - CaCl₂的血清中孵育时，C3转化酶也具有活性。通过免疫荧光在Pb18细胞表面证实了补体成分C3及其片段C3c、C3d、C3g、因子H、因子B、C4和C5b - 9，尽管未观察到对真菌活力的影响。
