戊巴比妥钠对不同脑区乙酰胆碱表观周转率的影响。

Effect of sodium pentobarbital on the apparent turnover of acetylcholine in different brain regions.

作者信息

Nordberg A, Sundwall A

出版信息

Acta Physiol Scand. 1977 Mar;99(3):336-44. doi: 10.1111/j.1748-1716.1977.tb10386.x.

DOI:10.1111/j.1748-1716.1977.tb10386.x

Abstract

The turnover rate of acetylcholine(ACh)was measured in six different brain regions in the mouse following pulse injection of radioactive choline (Ch) and killing of the animals by microwave irradiation of the head (0.25 s, 5 kW). The time course of the change in 3H-ACh/3H-Ch ratio was linear 0-06 s in all brain regions after administration of 3H-Ch. Plots of the specific radioactivities (SA) of ACh and Ch versus time indicate a precursor-product relationship in all brain regions except the cerebellum both in control and sodium pentobarbital anesthesized animals. The turnover was highest in the striatum (55 nmol-g-1-min-1), while in the cortex and hippocampus this value was approximately half (27 and 21 nmol-g-1-min-1). In the midbrain and medulla oblongata the turnover rates were only 11 and 10 nmol-g-1-min-1. Sodium pentobarbital anesthesia reduced specifically the turnover in the cortex and hippocampus to about 60 to 70 percent.

摘要

在给小鼠脉冲注射放射性胆碱（Ch）并用头部微波辐射（0.25秒，5千瓦）处死动物后，测量了小鼠六个不同脑区中乙酰胆碱（ACh）的周转率。给予³H-Ch后，所有脑区中³H-ACh/³H-Ch比值变化的时间进程在0至0.06秒内呈线性。在对照动物和戊巴比妥钠麻醉的动物中，除小脑外，所有脑区中ACh和Ch的比放射性（SA）与时间的关系图均显示出前体-产物关系。纹状体中的周转率最高（55 nmol·g⁻¹·min⁻¹），而在皮质和海马体中，该值约为其一半（27和21 nmol·g⁻¹·min⁻¹）。在中脑和延髓中，周转率仅为11和10 nmol·g⁻¹·min⁻¹。戊巴比妥钠麻醉特异性地将皮质和海马体中的周转率降低至约60%至70%。