Modak A T, Weintraub S T, McCoy T H, Stavinoha W B
J Pharmacol Exp Ther. 1976 May;197(2):245-52.
Microwave irradiation of 6 kw at 2450 MHz for 300 msec was sufficient to completely inactivate mouse brain cholinesterase and choline acetyltransferase. After this method of sacrifice, the acetylcholine contents of mouse brain regions, given in nanomoles per gram, were found to be: striatum, 81; medulla-pons, 44; diencephalon-midbrain, 34; hippocampus, 31; cerebral cortex, 26; and cerebellum, 17. Sodium pentobarbital caused a dose-dependent increase in whole brain acetylcholine. A maximal increase of 81% in whole brain was seen at 15 minutes with 80 mg/kg of sodium pentobarbital. The increase in acetylcholine after sodium pentobarbital treatment was not caused by anoxia from respiratory depression or by hypothermia. All brain regions except the cerebellum exhibited an increase in acetylcholine after pentobarbital treatment. Fifteen minutes after treatment, cerebellar acetylcholine was significantly decreased. However, at the time when half of the animals had regained the righting reflex, the unconscious mice showed an increase in cerebellar acetylcholine which was statistically significant as compared to control. The relative accumulation rate of acetylcholine calculated for cerebral cortex and hippocampus was higher than that for striatum although the absolute rate of accumulation of ACh was higher in the striatum. Thus, after sodium pentobarbital treatment, the cerebral cortex and hippocampus exhibit a greater cholinergic response than the striatum.
在2450兆赫频率下以6千瓦的功率进行300毫秒的微波辐射足以完全灭活小鼠脑胆碱酯酶和胆碱乙酰转移酶。采用这种处死后，发现小鼠脑各区域以每克纳摩尔计的乙酰胆碱含量分别为：纹状体，81；延髓-脑桥，44；间脑-中脑，34；海马体，31；大脑皮层，26；小脑，17。戊巴比妥钠可使全脑乙酰胆碱呈剂量依赖性增加。给予80毫克/千克戊巴比妥钠，15分钟时全脑乙酰胆碱含量最高可增加81%。戊巴比妥钠治疗后乙酰胆碱的增加并非由呼吸抑制导致的缺氧或体温过低引起。除小脑外，所有脑区在戊巴比妥钠治疗后乙酰胆碱均有增加。治疗15分钟后，小脑乙酰胆碱显著降低。然而，在一半动物恢复翻正反射时，未清醒小鼠的小脑乙酰胆碱与对照组相比有统计学意义的增加。尽管纹状体中乙酰胆碱的绝对积累速率较高，但计算得出大脑皮层和海马体中乙酰胆碱的相对积累速率高于纹状体。因此，戊巴比妥钠治疗后，大脑皮层和海马体比纹状体表现出更大的胆碱能反应。
