Herrero J F, Coates T W, Higgins M, Livingston A, Waterman A E, Headley P M
Department of Physiology, University of Bristol, School of Medical Sciences, UK.
J Neurosci Methods. 1993 Mar;46(3):225-32. doi: 10.1016/0165-0270(93)90071-x.
A technique is described for implanting a chamber on 1 or 2 vertebrae of the spinal column of the sheep. This chamber protrudes permanently through the dorsal skin of the back and is covered by a light bandage. Between recording sessions the chamber houses an inner cap sealing the hole that gives access to the cord. During recording sessions this cap is removed and a miniature manipulator inserted instead. This manipulator can accept a motor drive that holds a glass-coated tungsten microelectrode. The drive has a hole through which an arthroscope tube can be passed so that insertion of the electrode can be performed under visual control. Extracellular recordings have been made of single spinal neurones for up to 4 h in animals that are drug-free, untrained and only lightly restrained. Recording sessions can be repeated on a daily basis for several weeks until the dura and/or arachnoid becomes too thickened to permit electrode penetrations. Animals remain healthy and their behaviour remains normal throughout this time.
描述了一种在绵羊脊柱的1个或2个椎骨上植入腔室的技术。该腔室永久性地从背部的背部皮肤突出，并覆盖有轻质绷带。在记录间隙期间，腔室容纳一个密封通向脊髓的孔的内盖。在记录期间，取下该盖子，取而代之插入一个微型操纵器。该操纵器可以接受一个装有玻璃涂层钨微电极的电机驱动器。该驱动器有一个孔，关节镜管可以穿过该孔，以便在视觉控制下插入电极。在未用药、未训练且仅轻度约束的动物中，已经对单个脊髓神经元进行了长达4小时的细胞外记录。记录过程可以每天重复进行数周，直到硬脑膜和/或蛛网膜变得太厚而无法进行电极穿透。在此期间，动物保持健康，行为保持正常。
