[Effective expression of genes for interleukin-2 and its mutant analogs in E. coli cells].
作者信息
Seregin S V, Daniliuk N K, Siniakov A N, Kamynina T P, Il'iukova L V, Sakhno L V
出版信息
Mol Biol (Mosk). 1993 Jan-Feb;27(1):72-80.
PMID:8483475
Abstract
Recombinant plasmids were constructed for the efficient expression in E. coli cells of the human interleukin-2 (HIL-2) gene and two its mutant analogues obtained by of chemical-enzymic synthesis and polymerase chain reaction (deletion of 14 C-terminal amino acids and a change of the codon for Trp121 to Phe). The recombinant HIL-2 but not the mutant analogues were shown to be biologically active. Both analogues obtained were weak antagonists to HIL-2.
摘要
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