一种用于在大肠杆菌和长双歧杆菌中生产人白细胞介素-10的新型二元表达载体。
A novel binary expression vector for production of human IL-10 in Escherichia coli and Bifidobacterium longum.
作者信息
Reyes Escogido M L, De León Rodríguez A, Barba de la Rosa A P
机构信息
Division of Molecular Biology, Institute for Scientific and Technological Research of San Luis Potosí, SLP, México, Camino a la Presa San José 2055, Col Lomas 4 sección, CP 78216, San Luis Potosi, SLP, Mexico.
出版信息
Biotechnol Lett. 2007 Aug;29(8):1249-53. doi: 10.1007/s10529-007-9376-8. Epub 2007 May 9.
A novel expression vector (pLR) driven by hup promoter and Bifidobacterium beta-galactosidase signal peptide was constructed. The pLR vector was used for the expression of the optimized human IL-10 synthetic gene in Escherichia coli and Bifidobacterium longum. In both microorganisms, rhIL-10 was in a soluble form in total extract cells. The recombinant hIL-10 was partially processed in E. coli, whereas in Bifidobacterium all rhIL-10 was found in the mature form.
构建了一种由hup启动子和双歧杆菌β-半乳糖苷酶信号肽驱动的新型表达载体（pLR）。pLR载体用于在大肠杆菌和长双歧杆菌中表达优化的人IL-10合成基因。在这两种微生物中，重组人IL-10（rhIL-10）在总提取细胞中均为可溶形式。重组hIL-10在大肠杆菌中部分加工，而在双歧杆菌中所有rhIL-10均以成熟形式存在。
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