Sakaki T, Wato M, Otake S, Shirasu R, Tanaka A
First Department of Oral and Maxillofacial Surgery, Osaka Dental University, Japan.
Acta Pathol Jpn. 1993 Mar;43(3):99-106. doi: 10.1111/j.1440-1827.1993.tb01117.x.
Immunohistochemical investigations were carried out on the localization and expression of the Ca(2+)-dependent intercellular adhesion molecule E-cadherin in human gingiva and gingival carcinoma. Although E-cadherin did not appear in the parakeratinized layer of either clinically healthy or inflamed gingiva, it did appear strongly in the prickle layer and somewhat more weakly in the basal layer. Immunogold particles reactive to anti-E-cadherin monoclonal antibody in the electron microscopic findings were localized only in the vicinity of the desmosomes of the prickle and basal layers. In the case of gingival carcinoma, although E-cadherin was strongly expressed in the cells surrounding the keratinized region in the cancer nests, the expression decreased towards the marginal portion of the cancer nests. The distribution of E-cadherin in these cells may be dependent on the condition of the cancer cells that are potentially invasive. These findings suggest that cells of the parakeratinized layer of gingiva and cells of the marginal portion of the gingival carcinoma nests may easily detach or invade. In addition, the findings suggest that the gingival carcinoma used in this study tended to be invasive.
对人牙龈和牙龈癌中钙依赖性细胞间粘附分子E-钙粘蛋白的定位和表达进行了免疫组织化学研究。虽然E-钙粘蛋白在临床健康或发炎牙龈的不全角化层中均未出现，但在棘层中强烈出现，在基底层中略显较弱。电子显微镜检查中，对抗E-钙粘蛋白单克隆抗体有反应的免疫金颗粒仅定位于棘层和基底层桥粒附近。在牙龈癌的情况下，虽然E-钙粘蛋白在癌巢中角化区域周围的细胞中强烈表达，但向癌巢边缘部分表达降低。这些细胞中E-钙粘蛋白的分布可能取决于潜在侵袭性癌细胞的状况。这些发现表明，牙龈不全角化层细胞和牙龈癌巢边缘部分的细胞可能容易脱离或侵袭。此外，这些发现表明本研究中使用的牙龈癌倾向于具有侵袭性。
