Barwise J L, Walker J H
Department of Biochemistry and Molecular Biology, University of Leeds, UK.
FEBS Lett. 1996 Sep 30;394(2):213-6. doi: 10.1016/0014-5793(96)00950-7.
Annexin V is a major intracellular calcium-binding protein in human foreskin fibroblasts. Immunocytochemistry revealed that annexin V was localized in the nucleus and throughout the cytoplasm in human foreskin fibroblasts. The presence of annexin V in the nucleus was variable depending on the growth state. Nuclear staining was strongest in proliferating cells immediately after sub-culture, and decreased on prolonged culture without changing the culture medium. The cytoplasmic location of annexin V was not greatly affected by the same conditions. Refeeding cells with fresh serum restored annexin V to the nuclei of all cells within 24 h indicating that nuclear localization of annexin V is dependent on serum factors.
膜联蛋白V是人类包皮成纤维细胞中的一种主要细胞内钙结合蛋白。免疫细胞化学显示，膜联蛋白V定位于人类包皮成纤维细胞的细胞核及整个细胞质中。细胞核中膜联蛋白V的存在情况因生长状态而异。在传代培养后立即增殖的细胞中，核染色最强，在不更换培养基的情况下长时间培养后核染色会减弱。相同条件对膜联蛋白V在细胞质中的定位影响不大。用新鲜血清重新培养细胞可在24小时内使所有细胞的细胞核中恢复膜联蛋白V，这表明膜联蛋白V的核定位依赖于血清因子。
