Lu D, Das D K
Department of Surgery, University of Connecticut School of Medicine, Farmington 06030-1110.
Biochem Biophys Res Commun. 1993 Apr 30;192(2):808-12. doi: 10.1006/bbrc.1993.1486.
Amphetamine, a sympathomimetic drug, can elevate the body temperature by causing enhancement of endogenous lipolysis. This study was designed to examine whether this drug could induce the expression of the genes of heat shock proteins (HSP) in different tissues. Subcutaneous administration of amphetamine (10 mg/kg wt) to the rats increased the rectal temperature of the rats to 42 degrees C within 60 min. After 3 hr rats were sacrificed; and heart, lung, liver, kidney and brain tissue were removed to examine the induction of mRNAs for HSPs by Northern blot analysis using cDNA probes of 27 kDa, 70 kDa and 89 kDa HSPs. The results of this study indicate striking regional and cell type differences in the pattern of induction of the HSP mRNAs by amphetamine suggesting that different organs and cell types respond differently to amphetamine.
苯丙胺是一种拟交感神经药物，可通过增强内源性脂肪分解来升高体温。本研究旨在检验该药物是否能诱导不同组织中热休克蛋白（HSP）基因的表达。给大鼠皮下注射苯丙胺（10毫克/千克体重），60分钟内大鼠直肠温度升至42摄氏度。3小时后处死大鼠；取出心脏、肺、肝脏、肾脏和脑组织，使用27千道尔顿、70千道尔顿和89千道尔顿热休克蛋白的cDNA探针，通过Northern印迹分析检测热休克蛋白mRNA的诱导情况。本研究结果表明，苯丙胺诱导热休克蛋白mRNA的模式存在显著的区域和细胞类型差异，这表明不同器官和细胞类型对苯丙胺的反应不同。
