Mahmoud Kamel Z, Edens F W, Eisen E J, Havenstein G B
Department of Poultry Science, North Carolina State University, Box 7635, Raleigh, NC 27695-7635, USA.
Comp Biochem Physiol C Toxicol Pharmacol. 2004 Jan;137(1):11-8. doi: 10.1016/j.cca.2003.10.013.
A 2 x 2 factorially arranged completely randomized experimental design was used to study the relationship between inorganic phosphorus (Pi) and heat shock protein (HSP) mRNA expression in different organs of commercial broiler cockerels during acute heat stress (HS). Brain, heart, liver and spleen were assayed for hsp70, hsp90alpha and hsp90beta mRNA. At 1 day of age, chickens were assigned randomly to two dietary Pi treatment groups (Pi-: 0.16%; Pi+: 0.5%). At 3 weeks of age, half of the chickens in each Pi group were subjected to HS (Ta=41 degrees C, 60 min) while the other half was maintained in a thermoneutral environment (CN, Ta=25 degrees C). The results showed inter-organ variation in the expression of HSP mRNAs. Brain expressed the most HSP mRNAs while spleen expressed the least. When broilers were subjected to HS, the expression of HSP mRNAs was influenced positively by the consumption of the Pi+ diet. However, analysis of variance revealed that Ta influenced HSP transcription more than phosphorus availability. Thermal stress caused induction of hsp90alpha and hsp90beta in heart, liver and spleen, but hsp90alpha and hsp9beta mRNA levels were stable in brain. Transcription of hsp70 increased (P< or =0.05) in all organs from chickens in HS groups when compared to chickens in CN groups. Although Pi+ did not show any significant increases in the expression of hsp mRNAs, there were consistently larger HSP mRNA values in liver and spleen tissues. The high expression of hsp90alpha and hsp90beta in brain of chicks in both CN and HS conditions could be due to the involvement of hsp90 in steroid hormone receptors or the high metabolic activity of neurons in the central nervous system.
采用2×2析因完全随机实验设计，研究急性热应激（HS）期间商品肉仔公鸡不同器官中无机磷（Pi）与热休克蛋白（HSP）mRNA表达之间的关系。检测脑、心、肝和脾中hsp70、hsp90α和hsp90β mRNA。1日龄时，将鸡随机分为两个日粮Pi处理组（Pi-：0.16%；Pi+：0.5%）。3周龄时，每个Pi组的一半鸡接受热应激（Ta = 41℃，60分钟），另一半鸡饲养在热中性环境（CN，Ta = 25℃）中。结果显示，HSP mRNA表达存在器官间差异。脑中表达的HSP mRNA最多，而脾中表达的最少。当肉鸡受到热应激时，Pi+日粮的摄入对HSP mRNA的表达有正向影响。然而，方差分析表明，Ta对HSP转录的影响大于磷的可利用性。热应激导致心脏、肝脏和脾脏中hsp90α和hsp90β的诱导，但hsp90α和hsp9β mRNA水平在脑中稳定。与CN组鸡相比，HS组鸡所有器官中hsp70的转录均增加（P≤0.05）。虽然Pi+在hsp mRNA表达上没有显示出任何显著增加，但肝脏和脾脏组织中的HSP mRNA值始终较大。在CN和HS条件下，雏鸡脑中hsp90α和hsp90β的高表达可能是由于hsp90参与类固醇激素受体或中枢神经系统中神经元的高代谢活性。
