Guo X Z, Liu Y J, Wang Y L, Xu R B
Department of Pathophysiology, Second Military Medical University, Shanghai.
Sheng Li Xue Bao. 1997 Aug;49(4):370-4.
In order to address the question whether stress in intact higher animals may induce cellular heat shock response in distal organs, the inhibition of normal gene expression was studied on the basis of our previous findings about the induction of heat shock proteins in liver and brain of rats after scalding. Male SD rats were scalded on the back, 10-240 min thereafter decapitated, and the heat shock suppressed gene-1 was quantitated by dot blotting. The results showed that gene-1 decreased rapidly after scalding in both the organs, and did not recover to control levels even 240 min after scalding. The decrease of gene-1 went parallell with the severity of scalding. Thus it may be concluded that stress may induce heat shock response of distant organs in intact animals. Possible pathological significance of these findings was discussed.
为了探讨完整高等动物所受应激是否会在远端器官诱导细胞热休克反应，基于我们之前关于烫伤后大鼠肝脏和大脑中热休克蛋白诱导的研究结果，对正常基因表达的抑制情况进行了研究。雄性SD大鼠背部烫伤，10 - 240分钟后断头，通过斑点杂交法定量热休克抑制基因-1。结果显示，烫伤后两个器官中的基因-1均迅速下降，甚至在烫伤240分钟后仍未恢复到对照水平。基因-1的下降与烫伤的严重程度平行。因此可以得出结论，应激可能会在完整动物的远端器官诱导热休克反应。并对这些发现可能的病理意义进行了讨论。
