Beazley L D, Darby J E
Department of Psychology, University of Western Australia, Nedlands.
Vis Neurosci. 1993 Mar-Apr;10(2):297-301. doi: 10.1017/s0952523800003692.
We have previously reported that during optic nerve regeneration in the frog, 30-40% of retinal ganglion cells die, the loss being complete within 10 weeks. In the present study, we crushed the optic nerve, waited 10 weeks, and then recrushed the nerve at the same site. Retinae were examined 10 weeks later. We estimated ganglion cell numbers from cresyl-violet-stained wholemounts and found a fall of 53% compared to normals. The loss was significantly greater than the losses of 36% and 35%, respectively, in frogs which received a single optic nerve crush and were examined 10 or 20-24 weeks later. The results indicate that a second episode of ganglion cell death took place when the optic nerve regenerated a second time. We conclude that ganglion cells in the frog are not comprised of two subpopulations, only one of which intrinsically possesses the ability to regenerate.
我们之前曾报道过，在青蛙视神经再生过程中，30%-40%的视网膜神经节细胞会死亡，这种损失在10周内完成。在本研究中，我们压迫视神经，等待10周，然后在同一部位再次压迫神经。10周后检查视网膜。我们从甲酚紫染色的整装片中估计神经节细胞数量，发现与正常情况相比下降了53%。这种损失明显大于单次视神经压迫后10周或20-24周检查的青蛙分别出现的36%和35%的损失。结果表明，当视神经再次再生时，发生了第二轮神经节细胞死亡。我们得出结论，青蛙的神经节细胞并非由两个亚群组成，其中只有一个亚群内在地具备再生能力。
