St-Pierre B, Couture C, Laroche A, Pallotta D
Départment de Biologie, Faculté des Sciences et Génie, Université Laval, Québec, Canada.
Biochim Biophys Acta. 1993 Apr 29;1173(1):107-10. doi: 10.1016/0167-4781(93)90254-b.
A cDNA library from amoebae of Physarum polycephalum was screened by differential hybridization. Two clones contained inserts for mRNAs present in amoebae and absent in plasmodia. The LAV3-4 cDNA encodes a 402 aa protein (ABP-46) that shows sequence similarity to the actin binding site in the N-terminal region of the alpha-actinin family. The LAV3-5 cDNA is 76% identical to the Dictyostelium actin bundling protein, which cross-links and stabilizes actin filaments in amoebal filopodia.
