[蜡样芽孢杆菌磷脂酶C催化磷脂水解动力学的特征。不同聚集状态下磷脂酰肌醇的水解]

[Characteristics of phospholipid hydrolysis kinetics by phospholipase C from Bacillus cereus. Hydrolysis of phosphatidylinositol in various aggregated states].

作者信息

Selishcheva A A, Miroshnikova T A, Voronin M V, Vasilenko I A

出版信息

Biokhimiia. 1993 Mar;58(3):340-7.

Abstract

The process of phospholipase C hydrolysis of phosphatidylethanolamine (PE) in the form of mixed micelles phospholipid-detergent or in the form of vesicules in the mixture with phosphatidylcholine (PC) was studied. The size of the micelles was measured by dynamic light-scattering and their structure was determined by 31P-NMR spectroscopy. It was found that the kinetics of PE hydrolysis in the micelles by phospholipase C from Bacillus cereus do not follow Michaelis-Menten equation, but at all concentrations studied PE was hydrolyzed significantly slower than PC. The rate of PC hydrolysis was measured in the previous work. The hydrolysis of PE in PC-vesicules was followed with use of Victoria blue R dye method. It was shown that the rate of PE hydrolysis in the vesicules is similar to that of PC.

摘要

研究了磷脂酶C在磷脂-去污剂混合胶束形式或与磷脂酰胆碱（PC）混合的囊泡形式下对磷脂酰乙醇胺（PE）的水解过程。通过动态光散射测量胶束大小，并通过31P-NMR光谱确定其结构。发现蜡样芽孢杆菌的磷脂酶C对胶束中PE的水解动力学不符合米氏方程，但在所研究的所有浓度下，PE的水解速度明显慢于PC。PC水解速率已在先前的工作中测定。使用维多利亚蓝R染料法跟踪PC囊泡中PE的水解。结果表明，囊泡中PE的水解速率与PC相似。