Hansen M B, Svenson M, Diamant M, Bendtzen K
Department of Infectious Diseases, Rigshospitalet University Hospital, Copenhagen, Denmark.
Cytokine. 1993 Jan;5(1):72-80. doi: 10.1016/1043-4666(93)90026-2.
Nano- to picomolar concentrations of high affinity IgG antibody to interleukin 6 (IL-6ab) were detected in sera of 71 of 467 normal Danish blood donors (15%). IL-6 bound to the Fab fragments of IL-6ab, and the antibody specifically and competitively interfered with enzyme-linked immunosorbent assays for human IL-6. Only IL-6ab-positive sera interfered with these ELISAs. A statistically positive correlation was found between total IL-6 binding and specific IL-6 binding to serum (P < 0.0001), suggesting that IL-6ab dominates as IL-6 binding factors in normal human serum. The IL-6ab also inhibited binding of IL-6 to receptors on the human U-937 macrophage-like cell line, the human U-266 myeloma cell line and the mouse hybridoma cell line B13.29, clone B9 (B9 cells). IL-6-induced proliferation of the B9 cells was competitively inhibited by IL-6ab. We conclude that sera of normal individuals may contain appreciable levels of autoantibodies to IL-6. These IgG autoantibodies may be physiologically and pathophysiologically important because they are potent inhibitors of IL-6 in vitro.
在467名丹麦正常献血者的血清中，有71人（15%）检测到纳摩尔至皮摩尔浓度的高亲和力白细胞介素6抗体（IL-6ab）。IL-6与IL-6ab的Fab片段结合，该抗体特异性地、竞争性地干扰人IL-6的酶联免疫吸附测定。只有IL-6ab阳性血清会干扰这些酶联免疫吸附测定。总IL-6结合与血清特异性IL-6结合之间存在统计学上的正相关（P < 0.0001），这表明IL-6ab作为正常人血清中IL-6结合因子占主导地位。IL-6ab还抑制IL-6与人U-937巨噬细胞样细胞系、人U-266骨髓瘤细胞系和小鼠杂交瘤细胞系B13.29（B9细胞）受体的结合。IL-6诱导的B9细胞增殖受到IL-6ab的竞争性抑制。我们得出结论，正常个体的血清可能含有相当水平的抗IL-6自身抗体。这些IgG自身抗体在体外是IL-6的有效抑制剂，可能在生理和病理生理方面具有重要意义。
