Milman N, Jacobsen G K, Faurschou P, Graudal N, Jørgensen A
Department of Pulmonary Medicine, Gentofte Hospital, University of Copenhagen, Denmark.
Cytopathology. 1993;4(2):85-9. doi: 10.1111/j.1365-2303.1993.tb00519.x.
The effect of filtration through two layers of cotton gauze on the cellular composition of bronchoalveolar lavage (BAL) fluid was studied in 25 patients with pulmonary disease. There was no significant difference between median total cell count in unfiltered and filtered BAL (P = 0.73) or in the distribution of neutrophils or eosinophils. The percentage of bronchial epithelial cells was significantly higher in unfiltered than in filtered BAL (P = 0.02). Furthermore, differential cell counts showed a significantly lower percentage of alveolar macrophages (P = 0.04), and a significantly higher percentage of lymphocytes (P = 0.04) in unfiltered compared with filtered BAL. Thus, gauze filtration results in a loss of bronchial epithelial cells and lymphocytes, and is not recommended in the routine analysis of cellular components in BAL fluid.
对25例肺部疾病患者研究了通过两层棉纱过滤对支气管肺泡灌洗（BAL）液细胞组成的影响。未过滤和过滤后的BAL液中总细胞计数中位数之间无显著差异（P = 0.73），中性粒细胞或嗜酸性粒细胞的分布也无显著差异。未过滤的BAL液中支气管上皮细胞的百分比显著高于过滤后的BAL液（P = 0.02）。此外，细胞分类计数显示，与过滤后的BAL液相比，未过滤的BAL液中肺泡巨噬细胞的百分比显著降低（P = 0.04），淋巴细胞的百分比显著升高（P = 0.04）。因此，纱布过滤会导致支气管上皮细胞和淋巴细胞丢失，不建议用于BAL液细胞成分的常规分析。
