De Brauwer Els I G B, Jacobs Jan A, Nieman Fred, Bruggeman Cathrien A, Drent Marjolein
Department of Medical Microbiology, Atrium Medical Centre Heerlen, Heerlen, The Netherlands.
Anal Quant Cytol Histol. 2002 Dec;24(6):337-41.
To investigate the number of cells to be counted in cytocentrifuged bronchoalveolar lavage (BAL) fluid preparations in order to reach a reliable enumeration of each cell type.
A total of 136 BAL fluid samples for patients with suspected pneumonia or interstitial lung disease were investigated. Differential cell counts were performed on May-Grünwald-Giemsa-stained cytocentrifuged preparations by 2 observers, each differentiating 500 cells. Reliability for the enumeration of each cell type was expressed as phi value, as calculated in generalizability theory.
For polymorphonuclear neutrophils (PMNs), alveolar macrophages, lymphocytes and eosinophils, an acceptable phi value of > or = .95 was reached at a count of 300 cells by 1 observer. Mast cells reached a phi value of only .674 at a count of 500 cells by 1 observer, precluding a reliable count. At a count of 500 cells by 1 observer, squamous epithelial cells, bronchial epithelial cells and plasma cells displayed phi values of .868, .903 and .816, respectively.
At a count of 300 cells, PMNs, alveolar macrophages, lymphocytes and eosinophils are reliably enumerated in cytocentrifuged BAL fluid samples.
研究细胞离心法处理的支气管肺泡灌洗（BAL）液标本中需要计数的细胞数量，以便对每种细胞类型进行可靠的计数。
共调查了136例疑似肺炎或间质性肺疾病患者的BAL液样本。两名观察者对May-Grünwald-Giemsa染色的细胞离心涂片进行细胞分类计数，每人辨别500个细胞。每种细胞类型计数的可靠性用概化理论计算的phi值表示。
对于多形核中性粒细胞（PMN）、肺泡巨噬细胞、淋巴细胞和嗜酸性粒细胞，一名观察者计数300个细胞时，phi值达到可接受的≥0.95。一名观察者计数500个细胞时，肥大细胞的phi值仅为0.674，无法进行可靠计数。一名观察者计数500个细胞时，鳞状上皮细胞、支气管上皮细胞和浆细胞的phi值分别为0.868、0.903和0.816。
计数300个细胞时，可对细胞离心法处理的BAL液样本中的PMN细胞、肺泡巨噬细胞、淋巴细胞和嗜酸性粒细胞进行可靠计数。
