Nice E C, Fabri L, Hammacher A, Andersson K, Hellman U
Ludwig Institute for Cancer Research, Melbourne Tumour Biology Branch, Victoria, Australia.
Biomed Chromatogr. 1993 Mar-Apr;7(2):104-11. doi: 10.1002/bmc.1130070212.
Data on the characterization of anion and cation micropreparative (50 x 1.6 mm i.d.) HPLC columns is presented. It is shown how subnanomole quantities of protein can be efficiently recovered from such columns, rendering them compatible for use in multidimensional chromatographic strategies for the purification of trace biological samples. By selection of appropriate solvent systems (e.g., buffer-free sodium chloride solutions), the small eluant peak volumes can be loaded directly onto the gas phase sequencer, and N-terminal sequence data obtained. The potential of the technique is illustrated for the purification of a GTPase activating protein (GAP-3).
本文介绍了阴离子和阳离子微量制备型（内径50×1.6 mm）高效液相色谱柱的特性数据。展示了如何从这些柱子中高效回收亚纳摩尔量的蛋白质，使其适用于多维色谱策略，用于纯化痕量生物样品。通过选择合适的溶剂系统（例如，无缓冲氯化钠溶液），可以将小体积的洗脱峰直接加载到气相测序仪上，并获得N端序列数据。该技术的潜力通过纯化一种GTP酶激活蛋白（GAP - 3）得到了说明。
