Higo K, Higo H
Department of Molecular Biology, National Institute of Agrobiological Resources, Ibaraki, Japan.
Biochem Mol Biol Int. 1993 Mar;29(3):403-9.
In order to examine whether changes in gene expression are induced by injury in seedlings of rice (Oryza sativa) after a lethal freeze-thaw stress. mRNAs were extracted and their in vitro translation products were compared with those of the control plants by polyacrylamide gel electrophoresis. An mRNA encoding an entirely new protein with M(r) of ca. 37 kDa was detected as early as 30 min of thawing at 28 degrees C. Two-dimensional gel electrophoresis revealed the induction of mRNAs for at least 10 additional proteins, including those in a range of 15 to 20 kDa. Most of these proteins appear to be distinct from heat-shock proteins. The steady state level of mRNAs encoding putative major light-harvesting chlorophyll a/b binding protein-I and -II were, on the other hand, very low as compared with those of the control plants. The changes observed resemble those previously seen in cold-sensitive plants exposed to sub-lethal chilling temperatures (4-10 degrees C).
为了检测致死性冻融胁迫后水稻（Oryza sativa）幼苗中的基因表达变化是否由损伤诱导，提取了mRNA，并通过聚丙烯酰胺凝胶电泳将其体外翻译产物与对照植物的产物进行比较。早在28℃解冻30分钟时就检测到一种编码全新蛋白质（分子量约为37 kDa）的mRNA。二维凝胶电泳显示至少另外10种蛋白质的mRNA被诱导，包括分子量在15至20 kDa范围内的蛋白质。这些蛋白质中的大多数似乎与热休克蛋白不同。另一方面，与对照植物相比，编码假定的主要捕光叶绿素a/b结合蛋白-I和-II的mRNA的稳态水平非常低。观察到的变化类似于先前在暴露于亚致死低温（4-10℃）的冷敏感植物中看到的变化。
