Ramani S, Apte S K
Nuclear Agriculture Division, Bhabha Atomic Research Centre, Trombay, Mumbai, India.
Biochem Biophys Res Commun. 1997 Apr 28;233(3):663-7. doi: 10.1006/bbrc.1997.6521.
To date only about 15 abundantly expressed osmoresponsive genes/proteins have been described in rice cultivars. Using in vivo radiolabeling followed by two-dimensional electrophoresis and autoradiography, a record number of at least 35 salinity stress-induced polypeptides (14-90 kDa) and 17 salt stress-repressed polypeptides were detected in a halotolerant Indica rice cultivar Bura Rata. These include > 20 hitherto unreported rice polypeptides that exhibited a low abundance, short-term expression during NaCl stress. Prolonged exposure to NaCl decreased their synthesis. These findings have widened the scope of further investigations into new osmoresponsive genes, especially those with possibly transient regulatory functions in rice halotolerance.
迄今为止，在水稻品种中仅描述了约15个大量表达的渗透响应基因/蛋白质。通过体内放射性标记，然后进行二维电泳和放射自显影，在耐盐籼稻品种Bura Rata中检测到创纪录数量的至少35种盐胁迫诱导的多肽（14 - 90 kDa）和17种盐胁迫抑制的多肽。其中包括20多种迄今未报道的水稻多肽，它们在NaCl胁迫期间丰度较低、表达短暂。长时间暴露于NaCl会降低它们的合成。这些发现拓宽了对新的渗透响应基因进一步研究的范围，特别是那些在水稻耐盐性中可能具有瞬时调节功能的基因。
