Mercan D, Delville J P, Leclercq-Meyer V, Malaisse W J
Laboratory of Experimental Medicine, Brussels Free University, Belgium.
Biochem Mol Biol Int. 1993 Mar;29(3):475-81.
In purified pancreatic islet B cells, a rise in D-glucose concentration from 2.8 to 16.7 mM increased the production of both 14CO2 from D-[3,4-14C]glucose and 3HOH from D-[5-3H]glucose to a much greater relative extent than in purified non-B islet cells. Moreover, the paired ratio between D-[3,4-14C]glucose oxidation and D-[5-3H]glucose utilization was significantly increased, as a result of the rise in hexose concentration, in purified B cells, but not so in purified non-B cells. It is proposed, therefore, that a preferential stimulation by D-glucose of oxidative relative to total glycolysis represents an intrinsic attribute of insulin-producing cells, as distinct from other endocrine islet cells.
在纯化的胰岛B细胞中，D-葡萄糖浓度从2.8 mM升高至16.7 mM时，D-[3,4-¹⁴C]葡萄糖生成¹⁴CO₂以及D-[5-³H]葡萄糖生成³H₂O的量，相比于纯化的非B胰岛细胞，在相对程度上有更大幅度的增加。此外，由于己糖浓度升高，纯化B细胞中D-[3,4-¹⁴C]葡萄糖氧化与D-[5-³H]葡萄糖利用之间的配对比率显著增加，而纯化的非B细胞则不然。因此有人提出，与其他内分泌胰岛细胞不同，D-葡萄糖对氧化相对于总糖酵解的优先刺激是胰岛素产生细胞的固有特性。
