Koegler-Muly S M, Owens M J, Ervin G N, Kilts C D, Nemeroff C B
Department of Pharmacology, Duke University Medical Center, Durham, North Carolina 27710.
J Neuroendocrinol. 1993 Feb;5(1):95-8. doi: 10.1111/j.1365-2826.1993.tb00367.x.
The projection fields of corticotropin-releasing factor (CRF)-containing perikarya in the rat central nervous system were studied using a combination of electrolytic lesions, microdissection and radioimmunoassay. The effects of bilateral electrolytic lesions of the central nucleus of the amygdala (Ce) or the paraventricular nucleus (PVN) of the hypothalamus were measured by a sensitive and specific radioimmunoassay. Following lesions of the Ce, CRF concentrations in the locus ceruleus (LC) were significantly decreased. Following lesions of the PVN, CRF concentrations in the median eminence were markedly decreased (> 85%), with smaller but consistent reductions of CRF in the hippocampus as well. In contrast to the Ce lesions, PVN lesions resulted in increases in CRF concentrations in the LC. These results further confirm the projection of CRF-containing cells from the PVN to the median eminence, provide evidence for a PVN-hippocampal CRF pathway, and suggest that the PVN modulates CRF neurons innervating the LC. Moreover, the existence of a CRF-containing pathway from the Ce to the LC appears likely, and such a circuit may play a role in the behavioral and physiological responses to stress.
采用电解损伤、显微解剖和放射免疫测定相结合的方法，研究了大鼠中枢神经系统中含促肾上腺皮质激素释放因子（CRF）的神经元胞体的投射区域。通过灵敏且特异的放射免疫测定法，检测了杏仁核中央核（Ce）或下丘脑室旁核（PVN）双侧电解损伤的影响。Ce损伤后，蓝斑（LC）中的CRF浓度显著降低。PVN损伤后，正中隆起中的CRF浓度显著降低（>85%），海马中的CRF也有较小但一致的降低。与Ce损伤相反，PVN损伤导致LC中CRF浓度升高。这些结果进一步证实了含CRF的细胞从PVN向正中隆起的投射，为PVN-海马CRF通路提供了证据，并表明PVN调节支配LC的CRF神经元。此外，从Ce到LC的含CRF通路似乎存在，这样的回路可能在对应激的行为和生理反应中起作用。
