Slikkerveer A, Helmich R B, de Wolff F A
Toxicology Laboratory, University Hospital Leiden, The Netherlands.
Clin Chem. 1993 May;39(5):800-3.
We developed a simple method for the analysis of bismuth (Bi) in biological tissue, using wet digestion for sample pretreatment. Bi was determined by electrothermal atomic absorption spectrometry with platinum as a matrix modifier to decrease the volatility of Bi. The furnace program included a gas stop for sensitivity enhancement. Analytical performance was established for Bi in kidney, liver, brain, and bone. As little as 25 ng/g wet weight can be detected in the most concentrated digests. Homogenization of bone was necessary before digestion, and its matrix showed the strongest interference. In rats exposed orally to colloidal bismuth subcitrate for 14 days, the metal could be detected in liver, kidney, and spleen but not in brain and bone. In the tissues of 12 patients who died from non-Bi-related causes, no Bi were present in kidney in the other 2.
我们开发了一种用于分析生物组织中铋（Bi）的简单方法，采用湿法消解进行样品预处理。通过电热原子吸收光谱法，以铂作为基体改进剂来测定铋，以降低铋的挥发性。炉程序包括用于提高灵敏度的气体停止步骤。确定了该方法对肾脏、肝脏、脑和骨骼中铋的分析性能。在最浓缩的消解液中，最低可检测到25 ng/g湿重。消解前对骨骼进行匀浆是必要的，其基体显示出最强的干扰。在经口给予大鼠胶体次枸橼酸铋14天的实验中，在肝脏、肾脏和脾脏中可检测到该金属，但在脑和骨骼中未检测到。在12例因非铋相关原因死亡的患者组织中，另外2例患者的肾脏中未检测到铋。
