Tsanaclis L M, Wilson J F
Cardiff Bioanalytical Services Ltd., Cardiff Business Technology Centre, UK.
Clin Chem. 1993 May;39(5):851-5.
We compared the intra- and interlaboratory precision of seven techniques used to measure eight antiepileptic drugs, digoxin, and theophylline by using data from the international Healthcontrol external quality-assessment scheme. Scheme participants were supplied blind with 6 or 12 sets of duplicate lyophilized serum samples. Each set contained different drug concentrations, and duplicates were analyzed separately, 1 to 6 months apart. The intra- and interlaboratory components of assay variance were isolated and compared by Bartlett's test for homogeneity of variance. Fluorescence polarization immunoassay (Abbott) showed the best overall intra- and interlaboratory performance for a range of analytes. The largest intralaboratory errors were produced by techniques using the Syva EMIT assays. Our analysis of the data shows that for most analyte/technique combinations, intralaboratory sources of variation were more important than interlaboratory sources. Gains in assay precision will therefore result from attention to internal laboratory procedures.
我们利用国际健康控制外部质量评估计划的数据，比较了用于测量8种抗癫痫药物、地高辛和茶碱的7种技术在实验室内和实验室间的精密度。该计划的参与者被盲目提供6或12组重复的冻干血清样本。每组包含不同的药物浓度，重复样本分别进行分析，间隔1至6个月。通过Bartlett方差齐性检验分离并比较了分析方法差异的实验室内和实验室间成分。荧光偏振免疫分析（雅培公司）对一系列分析物显示出最佳的整体实验室内和实验室间性能。使用Syva EMIT分析方法的技术产生了最大的实验室内误差。我们对数据的分析表明，对于大多数分析物/技术组合，实验室内变异来源比实验室间变异来源更重要。因此，关注实验室内部程序将提高分析方法的精密度。
