Wilgenbus K K, Milatovich A, Francke U, Furthmayr H
Department of Pathology, Stanford University School of Medicine, California 94305.
Genomics. 1993 Apr;16(1):199-206. doi: 10.1006/geno.1993.1159.
Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. Recent cloning of the murine and porcine radixin cDNAs revealed a protein highly homologous to ezrin and moesin. We have cloned and sequenced the human radixin cDNA and found the predicted amino acid sequence for the human protein to be nearly identical to those predicted for radixin in the two other species. By Southern analyses of Chinese hamster x human somatic cell hybrid DNA and of PCR products derived from hybrids, the coding gene (RDX) was mapped to 11q. Fluorescence chromosomal in situ hybridization with a cDNA plasmid further localized this gene to band 11q23. However, PCR amplification with "radixin-specific" primers on the hybrid DNA panel yielded an additional, very similar DNA sequence that was further characterized by direct sequencing of PCR products. This sequence represents a truncated version and the respective locus (RDXP2) was assigned to Xp21.3. Furthermore, by employing a different set of primers, a third sequence was found that was 90% identical to the radixin sequence but contained termination codons and seemed to lack introns. This pseudogene (RDXP1) was mapped to 11p by Southern and PCR analyses.
根蛋白是一种细胞骨架蛋白，可能在将肌动蛋白连接到质膜上起重要作用。最近对小鼠和猪根蛋白cDNA的克隆揭示了一种与埃兹蛋白和膜突蛋白高度同源的蛋白质。我们已经克隆并测序了人类根蛋白cDNA，发现该人类蛋白质的预测氨基酸序列与另外两个物种中根蛋白的预测序列几乎相同。通过对中国仓鼠×人类体细胞杂种DNA以及来自杂种的PCR产物进行Southern分析，将编码基因（RDX）定位到11q。用cDNA质粒进行荧光染色体原位杂交进一步将该基因定位到11q23带。然而，在杂种DNA面板上用“根蛋白特异性”引物进行PCR扩增产生了另一个非常相似的DNA序列，通过对PCR产物进行直接测序对其进行了进一步表征。该序列代表一个截短版本，相应的基因座（RDXP2）被定位到Xp21.3。此外，通过使用另一组引物，发现了第三个序列，它与根蛋白序列有90%的同一性，但含有终止密码子，似乎缺乏内含子。通过Southern和PCR分析将这个假基因（RDXP1）定位到11p。
