20号染色体的多态性简单序列重复（SSR）标记
Polymorphic SSR (simple-sequence-repeat) markers for chromosome 20.
作者信息
Melis R, Bradley P, Elsner T, Robertson M, Lawrence E, Gerken S, Albertsen H, White R
机构信息
Eccles Institute for Human Genetics, University of Utah, Salt Lake City 84112.
出版信息
Genomics. 1993 Apr;16(1):56-62. doi: 10.1006/geno.1993.1140.
Chromosome 20-specific simple-sequence-repeat (SSR) markers were developed from a flow-sorted phage library (LL20NS01), subcloned in Bluescript, and screened with a tetranucleotide repeat, (AAAG)6, to identify potentially polymorphic loci. Of 100 clones sequenced, 39 were selected to construct primers. Of these 39, 22 were polymorphic. Reference to the CEPH linkage database (version 5) permitted genetic mapping of 16 of the new markers to specific regions of chromosome 20. Ten of the SSRs showed heterozygosity indices (above 70%) that would qualify them as potential index markers.
20号染色体特异性简单序列重复（SSR）标记是从一个经流式细胞仪分选的噬菌体文库（LL20NS01）中开发出来的，该文库亚克隆到蓝思载体中，并用四核苷酸重复序列（AAAG）6进行筛选，以鉴定潜在的多态性位点。在对100个克隆进行测序后，选择了39个来构建引物。在这39个中，有22个具有多态性。参考CEPH连锁数据库（第5版）可将16个新标记进行基因定位到20号染色体的特定区域。其中10个SSR显示出杂合度指数（高于70%），这使它们有资格成为潜在的索引标记。
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