包含微卫星重复序列的人类3号染色体基因座的物理图谱和遗传图谱

Physical and genetic mapping of human chromosome 3 loci containing microsatellite repeats.

作者信息

Gerken S, Whisenant E, Varkony T, Todd S, Gemmill R, Jones C, Weissenbach J, Matsunami N, Moore M, Lawrence E

机构信息

Division of Medical Oncology, University of Colorado Health Science Center, Denver, 80262.

出版信息

Chromosome Res. 1994 Nov;2(6):423-7. doi: 10.1007/BF01552864.

DOI:10.1007/BF01552864

PMID:7834219

Abstract

One hundred and six microsatellite repeat-containing loci, including 59 CA-containing repeats from the CEPH/Genethon collection, were regionally assigned on human chromosome 3 using a somatic cell hybrid mapping panel, diving the chromosome into 14 intervals. The others were dinucleotide and tetranucleotide repeat-containing loci newly developed for human chromosome 3, of which 26 were also localized by means of genetic linkage analysis against selected CEPH microsatellites. The regional assignment of these two marker sets in a common mapping panel facilitates their integration. Incorporation of these highly polymorphic loci into the developing physical and genetic maps should provide useful information for studies of various diseases involving chromosome 3.

摘要

利用体细胞杂种定位板，将106个含微卫星重复序列的位点（包括来自CEPH/Genethon文库的59个含CA重复序列）定位到人类3号染色体上，将该染色体划分为14个区间。其他位点是为人类3号染色体新开发的含二核苷酸和四核苷酸重复序列的位点，其中26个位点也通过与选定的CEPH微卫星进行遗传连锁分析进行了定位。在一个共同的定位板中对这两组标记进行区域定位有助于它们的整合。将这些高度多态性的位点纳入正在构建的物理图谱和遗传图谱中，应该能为涉及3号染色体的各种疾病的研究提供有用信息。