Ostrander E A, Sprague G F, Rine J
Cell and Molecular Biology, Human Genome Center, Lawrence Berkeley Laboratory, California 94720.
Genomics. 1993 Apr;16(1):207-13. doi: 10.1006/geno.1993.1160.
A large block of simple sequence repeat (SSR) polymorphisms for the dog genome has been isolated and characterized. Screening of primary libraries by conventional hybridization methods as well as by screening of enriched marker-selected libraries led to the isolation of a large number of genomic clones that contained (CA)n repeats. The sequences of 101 clones showed that the size and complexity of (CA)n repeats in the dog genome were similar to those reported for these markers in the human genome. Detailed analysis of a representative subset of these markers revealed that most markers were moderately to highly polymorphic, with PIC values exceeding 0.70 for 33% of the markers tested. An association between higher PIC values and markers containing longer (CA)n repeats was observed in these studies, as previously noted for similar markers in the human genome. A list of primer sequences that tag each characterized marker is provided, and a comprehensive system of nomenclature for the dog genome is suggested.
犬基因组的一大组简单序列重复（SSR）多态性已被分离和鉴定。通过常规杂交方法筛选初级文库以及筛选富集的标记选择文库，导致分离出大量包含（CA）n重复序列的基因组克隆。101个克隆的序列表明，犬基因组中（CA）n重复序列的大小和复杂性与人类基因组中这些标记的报道相似。对这些标记的一个代表性子集的详细分析表明，大多数标记具有中度至高多态性，33%的测试标记的PIC值超过0.70。在这些研究中观察到较高的PIC值与包含较长（CA）n重复序列的标记之间存在关联，如先前在人类基因组中对类似标记的观察。提供了标记每个已鉴定标记的引物序列列表，并提出了犬基因组的综合命名系统。
