人精子特异性同工酶在移植到Scid-nu(str)小鼠体内的精原细胞瘤细胞中的差异表达。
Differential expression of a human sperm-specific isozyme in seminoma cells transplanted in Scid-nu(str) mice.
作者信息
Takatera H, Nomura T, Itatani H
机构信息
Department of Radiation Biology, Faculty of Medicine, Osaka University.
出版信息
Jpn J Cancer Res. 1993 Mar;84(3):215-8. doi: 10.1111/j.1349-7006.1993.tb02858.x.
An isozyme of human sperm-specific lactate dehydrogenase, LDH-X (C4) (EC 1.1.1.27), which is expressed only in differentiated germ cells (spermatozoa, spermatids and primary spermatocytes after midpachytene), appeared in the xenografted tumor cells of human seminoma and its metastatic lesions (lymph node and kidney) in scid (severe combined immunodeficiency)-nude(streaker) double mutant mice, though it was not expressed in the original tumor of the patient. The morphological pattern of seminoma cells also changed in the xenografts and metastatic lesions as in normal spermatogenesis. Thus, the human seminoma cells showed differential expression of the sperm-specific isozyme in parallel with their morphological changes. However, the sperm-specific isozyme disappeared in the mitotically dividing seminoma cells which were newly established from the LDH-X positive xenograft.
人精子特异性乳酸脱氢酶同工酶LDH-X（C4）（EC 1.1.1.27）仅在分化的生殖细胞（精子、精子细胞和粗线期中期后的初级精母细胞）中表达，它出现在严重联合免疫缺陷-裸（条纹）双突变小鼠体内人精原细胞瘤的异种移植肿瘤细胞及其转移病灶（淋巴结和肾脏）中，尽管在患者的原发肿瘤中不表达。精原细胞瘤细胞的形态模式在异种移植和转移病灶中也如正常精子发生过程那样发生了变化。因此，人精原细胞瘤细胞在形态变化的同时，精子特异性同工酶也出现了差异表达。然而，从LDH-X阳性异种移植中新建立的、处于有丝分裂状态的精原细胞瘤细胞中，精子特异性同工酶消失了。
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