Furihata C, Oguchi S, Matsushima T
Department of Molecular Oncology, University of Tokyo.
Jpn J Cancer Res. 1993 Mar;84(3):223-9. doi: 10.1111/j.1349-7006.1993.tb02860.x.
The possible tumor-promoting and tumor-initiating activities of p-methylcatechol and methylhydroquinone in the pyloric mucosa of male F344 rats were studied. Ornithine decarboxylase (ODC) and replicative DNA synthesis (RDS) were used as markers of tumor promotion and DNA single strand scission and unscheduled DNA synthesis (UDS) as markers of tumor initiation. The compounds were administered by gastric intubation and results were compared with those after administration of catechol. p-Methylcatechol at doses of 60 to 180 mg/kg body weight dose-dependently induced up to 20-fold increase in ODC activity and 9-fold increase in RDS with maxima 24 h after its administration, but it did not induce significant DNA single strand scission or UDS. Methylhydroquinone at a dose of 200 mg/kg body weight induced up to 6-fold increase in ODC activity 24 h, and 5-fold increase in RDS 16 h after its administration, but the induction was not dose-dependent. At a dose of 200 mg/kg body weight it induced DNA single strand scission, but not UDS. These results and previous findings show that the possible tumor-promoting activities of catechol are several times higher than those of p-methylcatechol and 10 times higher than those of methylhydroquinone.
研究了对甲基邻苯二酚和甲基氢醌在雄性F344大鼠幽门黏膜中可能的促肿瘤和引发肿瘤活性。鸟氨酸脱羧酶（ODC）和复制性DNA合成（RDS）用作肿瘤促进的标志物，DNA单链断裂和非程序性DNA合成（UDS）用作肿瘤引发的标志物。通过胃插管给予这些化合物，并将结果与给予邻苯二酚后的结果进行比较。体重剂量为60至180mg/kg的对甲基邻苯二酚剂量依赖性地诱导ODC活性增加高达20倍，RDS增加9倍，给药后24小时达到最大值，但它没有诱导显著的DNA单链断裂或UDS。体重剂量为200mg/kg的甲基氢醌给药后24小时诱导ODC活性增加高达6倍，16小时诱导RDS增加5倍，但诱导不呈剂量依赖性。在体重剂量为200mg/kg时，它诱导DNA单链断裂，但不诱导UDS。这些结果和先前的发现表明，邻苯二酚的可能促肿瘤活性比对甲基邻苯二酚高几倍，比甲基氢醌高10倍。
