Bertram P A, Buller C S, Stewart G C, Akagi J M
University of Kansas, Department of Microbiology, Lawrence 66045.
J Appl Bacteriol. 1993 Apr;74(4):460-6. doi: 10.1111/j.1365-2672.1993.tb05154.x.
Bacteria capable of utilizing the water-insoluble purified extracellular (1-->3)-beta-D-glucan (curdlan) from Cellulomonas flavigena strain KU by extracellular enzymes, were isolated and characterized. Enrichment cultures from a Winogradsky column were incubated anaerobically at 55 degrees C with curdlan as the sole source of carbon. Colonies surrounded by zones of clearing were selected from subcultures on solid curdlan media. One of the isolates was chosen for further study and identified by conventional methods, API-tests with calculation of similarity coefficients and ID-scores, estimation of mol% (G+C) and DNA-DNA liquid hybridization. The isolate is a facultatively anaerobic, facultatively thermophilic Bacillus sp. Identification at the species-level was not achieved. The isolate was characterized by some rare traits among bacilli, but it remains unresolved whether it defines a new taxon.
从黄纤维单胞菌KU菌株中利用胞外酶分解水不溶性纯化胞外（1→3）-β-D-葡聚糖（凝胶多糖）的细菌被分离并进行了特性鉴定。以凝胶多糖作为唯一碳源，从维诺格拉德斯基柱中富集培养物在55℃厌氧条件下培养。从固体凝胶多糖培养基上的传代培养物中挑选出被透明圈包围的菌落。选择其中一个分离株进行进一步研究，并通过常规方法、计算相似系数和ID分数的API测试、mol%（G+C）估计以及DNA-DNA液相杂交进行鉴定。该分离株是兼性厌氧、兼性嗜热芽孢杆菌属。未实现种水平的鉴定。该分离株具有芽孢杆菌中的一些罕见特征，但它是否定义了一个新的分类单元仍未解决。
