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Characterization of N-ethoxycarbonyl ethyl esters of amino acids by mass spectrometry.

作者信息

Huang Z H, Wang J, Gage D A, Watson J T, Sweeley C C, Husek P

机构信息

Department of Biochemistry, Michigan State University, East Lansing 48824-1319.

出版信息

J Chromatogr. 1993 Apr 16;635(2):271-81. doi: 10.1016/0021-9673(93)80370-n.

DOI:10.1016/0021-9673(93)80370-n
PMID:8486747
Abstract

Derivatization of amino acids by using ethyl chloroformate-ethanol-pyridine provides volatile products, N-ethoxycarbonyl amino acid ethyl esters (ECEEs), which are easily amenable to GC or GC-MS analysis. MS behavior of these compounds under electron-impact has been studied. The fragments observed in the spectra facilitate recognition of commonly occurring protein amino acids and characterization of unknown analogues.

摘要

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