通过胶束电动毛细管电泳法分离强心苷。

Separation of cardiac glycosides by micellar electrokinetic capillary electrophoresis.

作者信息

Gaus H J, Treumann A, Kreis W, Bayer E

机构信息

Institut für Organische Chemie, Universität Tübingen, Germany.

出版信息

J Chromatogr. 1993 Apr 16;635(2):319-27. doi: 10.1016/0021-9673(93)80375-i.

DOI:10.1016/0021-9673(93)80375-i

PMID:8486748

Abstract

The separation of mixtures of primary and secondary cardiac glycosides by micellar electrokinetic capillary electrophoresis modified by cyclodextrins, urea and sodium cholate proved to be suitable for the determination of these hydrophobic compounds. It was possible to distinguish the two anomeric cardenolides glucodigifucoside and glucodigiglucomethyloside with all three buffer systems. Electropherograms of crude plant cell extracts from Digitalis lanata were obtained with this method.

摘要

通过环糊精、尿素和胆酸钠修饰的胶束电动毛细管电泳分离一级和二级强心苷混合物，被证明适用于测定这些疏水性化合物。使用这三种缓冲系统都能够区分两种异头强心甾糖苷葡萄糖地吉福苷和葡萄糖二葡萄糖甲基苷。用该方法获得了毛花洋地黄粗植物细胞提取物的电泳图。