Lucy M C, De La Sota R L, Staples C R, Thatcher W W
Dairy Science Department, University of Florida, Gainesville 32611-0920.
J Dairy Sci. 1993 Apr;76(4):1014-27. doi: 10.3168/jds.S0022-0302(93)77429-9.
Interactions of dietary energy and fat with recombinant bST (sometribove) injections were tested for their effects on ovarian function. Lactating dairy cows were fed one of three diets differing in energy concentration (NEL) and percentage of DM of calcium salts of long-chain fatty acids: diet 1, 1.68 Mcal/kg and 0%; diet 2, 1.68 Mcal/kg and 2.2%; and diet 3, 1.78 Mcal/kg and 2.2%. Cows were injected daily with bST or saline during one of two 19-d interestrous periods (crossover design) in which ovarian follicles were measured by ultrasonography. The bST-treated cows produced more FCM and were in lower energy balance than saline-treated cows. Before d 12 (first follicular wave, estrus = d 0), bST-treated cows had more ovarian follicles in classes 1 (3 to 5 mm; cows on diet 3) or 2 (6 to 9 mm; cows on diets 1 and 2) than saline-treated cows. After d 12 (preovulatory follicular wave), numbers of follicles in different size classes were similar for bST-treated and saline-treated cows, but cows fed diet 2 had larger preovulatory follicles. Lower dietary energy and bST treatment were both associated with larger subordinate follicles. Ovarian follicles less than 10 mm were stimulated by bST, and calcium salts of long-chain fatty acids increased diameters of preovulatory follicles.
研究了日粮能量和脂肪与重组牛生长激素（sometribove）注射之间的相互作用对卵巢功能的影响。给泌乳奶牛饲喂三种日粮中的一种，这三种日粮的能量浓度（净能）和长链脂肪酸钙盐占干物质的百分比不同：日粮1，1.68兆卡/千克，0%；日粮2，1.68兆卡/千克，2.2%；日粮3，1.78兆卡/千克，2.2%。在两个19天的发情周期之一（交叉设计）中，每天给奶牛注射牛生长激素或生理盐水，在此期间通过超声检查测量卵巢卵泡。接受牛生长激素处理的奶牛比接受生理盐水处理的奶牛产奶量更高，但能量平衡更低。在第12天之前（第一个卵泡波，发情日=第0天），接受牛生长激素处理的奶牛在1类（3至5毫米；日粮3的奶牛）或2类（6至9毫米；日粮1和2的奶牛）中的卵巢卵泡比接受生理盐水处理的奶牛更多。在第12天之后（排卵前卵泡波），接受牛生长激素处理的奶牛和接受生理盐水处理的奶牛不同大小类别的卵泡数量相似，但饲喂日粮2的奶牛有更大的排卵前卵泡。较低的日粮能量和牛生长激素处理均与较大的次级卵泡有关。牛生长激素刺激直径小于10毫米的卵巢卵泡，长链脂肪酸钙盐增加排卵前卵泡的直径。
