Carrera-Zandomeni M, Zandomeni R, Esteban E N, Ferrer J F
School of Veterinary Medicine, University of Pennsylvania, Kennett Square 19348.
J Infect Dis. 1993 May;167(5):1021-6. doi: 10.1093/infdis/167.5.1021.
The 24-kDa band formed when sera of humans infected with human T cell lymphotropic virus type I (HTLV-1) were reacted with HTLV-I lysates in conventional Western blot (WB) assays was found to be composed of two immunologically unrelated proteins of 24- and 23-kDa. p24, but not p23, carries epitopes shared by the major core proteins of the other known transactivating C-type retroviruses. p23 is unrelated immunologically to the env and tax HTLV-I products but partly cross-reacts with HTLV-I p19. All HTLV-I and simian T cell leukemia virus type I sera tested reacted with p23. Reactivity with p23 was seen with some HTLV-I sera that did not react or reacted weakly with HTLV-I p24. No reactivity with p23 was seen among the 51 human HTLV-II sera tested nor among a large panel of control sera. Because of its type-specificity and strong immunogenicity, p23 provides a reliable serologic marker for the diagnosis of HTLV-I infection and for distinguishing between HTLV-I and -II.
在传统的蛋白质免疫印迹（WB）分析中，当人类I型嗜T细胞病毒（HTLV-1）感染者的血清与HTLV-1裂解物反应时形成的24 kDa条带，被发现由两种免疫不相关的蛋白质组成，分别为24 kDa和23 kDa。p24携带其他已知反式激活C型逆转录病毒主要核心蛋白共有的表位，而p23则不携带。p23在免疫上与HTLV-1的env和tax产物无关，但与HTLV-1的p19有部分交叉反应。所有测试的HTLV-1和I型猿猴T细胞白血病病毒血清均与p23反应。一些不与HTLV-1 p24反应或反应较弱的HTLV-1血清也与p23反应。在测试的51份人类HTLV-II血清以及大量对照血清中均未观察到与p23的反应性。由于其型特异性和强免疫原性，p23为HTLV-1感染的诊断以及区分HTLV-1和HTLV-II提供了可靠的血清学标志物。
