Lu X S, Bélec L, Pillot J
Unité d'Immunologie Microbienne, Institut Pasteur, Paris, France.
J Infect Dis. 1993 May;167(5):1189-92. doi: 10.1093/infdis/167.5.1189.
To study the specific local immune response in vaginal fluids, 19 women infected with human immunodeficiency virus (HIV) type 1 and 23 seronegative controls were selected. Vaginal fluids were tested by ELISA for total IgG, IgA, and IgM levels and for specific IgG, IgA, and IgM antibodies to gp160. Total IgG, IgA, and IgM concentrations were 6.8-, 5.0-, and 2.5-fold higher, respectively, in HIV-1-infected women than in controls, with a positive correlation between IgG and IgA levels. IgG or IgA antibodies or both to gp160 were detected in 12 subjects (63%), whereas no IgM antibodies to gp160 were found. Anti-gp160 IgG strongly predominated. Serum samples were available for 11 women whose total IgG vaginal levels strongly correlated with total IgG in sera. These results suggest that transudation of serumborne antibodies is the main source of gp160-specific antibodies in the vaginal fluid of HIV-1-infected women.
为研究阴道液中的特异性局部免疫反应，选取了19名感染1型人类免疫缺陷病毒（HIV）的女性和23名血清学阴性对照者。通过酶联免疫吸附测定（ELISA）检测阴道液中总IgG、IgA和IgM水平以及针对gp160的特异性IgG、IgA和IgM抗体。HIV-1感染女性的总IgG、IgA和IgM浓度分别比对照者高6.8倍、5.0倍和2.5倍，IgG和IgA水平之间呈正相关。在12名受试者（63%）中检测到针对gp160的IgG或IgA抗体或两者皆有，而未发现针对gp160的IgM抗体。抗gp160 IgG占主导地位。11名女性有血清样本，其阴道总IgG水平与血清总IgG高度相关。这些结果表明，血清源性抗体的渗出是HIV-1感染女性阴道液中gp160特异性抗体的主要来源。
