Huang C J, Fwu M L
Department of Agricultural Chemistry, National Taiwan University, Taipei, Republic of China.
J Nutr. 1993 May;123(5):803-10. doi: 10.1093/jn/123.5.803.
To investigate the effects of various dietary protein levels on tissue lipid peroxidation and antioxidative enzyme activities, four groups of Long-Evans male weanling rats were fed a 6, 8, 12 or 20% lactalbumin diet for 6 wk. The red blood cell in vitro spontaneous hemolysis values were 28.6, 24.9, 18.9 and 14.1% for the rats fed the 6, 8, 12 and 20% lactalbumin diets, respectively. The thiobarbituric acid-reactive substances (TBARS) in plasma, RBC, liver, kidney, muscle, lung, spleen and heart were lowest in the group fed the 20% lactalbumin diet. Rats fed the diet containing 12% lactalbumin had significantly greater TBARS in plasma, spleen, kidney and muscle compared with rats fed the 20% lactalbumin diet. Rats fed the 8% lactalbumin diet had markedly higher TBARS levels (P < 0.05) in all tissues examined than did those fed the 12% lactalbumin diet. The TBARS in all eight tissues of the rats fed the 6% lactalbumin diet were even higher than those of rats fed the 8% lactalbumin diet (P < 0.05). The activities of glutathione peroxidase, superoxide dismutase and glucose-6-phosphate dehydrogenase in RBC were gradually lowered (P < 0.05) when the dietary protein level was decreased stepwise. The activities of these antioxidative enzymes in liver also were reduced significantly when dietary protein level decreased from 20 to 12% and then to 8%. These enzyme activities in the rats fed the diet containing 6% lactalbumin generally were not different from those of the rats fed 8% lactalbumin.(ABSTRACT TRUNCATED AT 250 WORDS)
为研究不同膳食蛋白质水平对组织脂质过氧化和抗氧化酶活性的影响，将四组雄性长 Evans 断奶大鼠分别喂食含 6%、8%、12%或 20%乳白蛋白的饲料，持续 6 周。喂食 6%、8%、12%和 20%乳白蛋白饲料的大鼠，其红细胞体外自发溶血值分别为 28.6%、24.9%、18.9%和 14.1%。血浆、红细胞、肝脏、肾脏、肌肉、肺、脾脏和心脏中的硫代巴比妥酸反应物质（TBARS）在喂食 20%乳白蛋白饲料的组中最低。与喂食 20%乳白蛋白饲料的大鼠相比，喂食含 12%乳白蛋白饲料的大鼠血浆、脾脏、肾脏和肌肉中的 TBARS 显著更高。喂食 8%乳白蛋白饲料的大鼠在所有检测组织中的 TBARS 水平均明显高于喂食 12%乳白蛋白饲料的大鼠（P < 0.05）。喂食 6%乳白蛋白饲料的大鼠所有八个组织中的 TBARS 甚至高于喂食 8%乳白蛋白饲料的大鼠（P < 0.05）。当膳食蛋白质水平逐步降低时，红细胞中谷胱甘肽过氧化物酶、超氧化物歧化酶和葡萄糖-6-磷酸脱氢酶的活性逐渐降低（P < 0.05）。当膳食蛋白质水平从 20%降至 12%，再降至 8%时，肝脏中这些抗氧化酶的活性也显著降低。喂食含 6%乳白蛋白饲料的大鼠这些酶的活性通常与喂食 8%乳白蛋白饲料的大鼠无差异。（摘要截短于 250 字）
