应用染色体原位抑制杂交技术对急性早幼粒细胞白血病中t(15;17)易位的描绘
Delineation of translocation t(15; 17) in acute promyelocytic leukemia by chromosomal in situ suppression hybridization.
作者信息
Speicher M R, Jauch A, Parr A, Becher R
机构信息
University of Heidelberg, Institute for Human Genetics and Anthropology, Germany.
出版信息
Leuk Res. 1993 Apr;17(4):359-64. doi: 10.1016/0145-2126(93)90024-f.
In this study we demonstrate the feasibility of chromosomal in situ suppression (CISS) hybridization to detect the translocation t(15; 17) in metaphase spreads of patients with acute promyelocytic leukemia. Using DNA libraries from sorted human chromosomes 15 and 17 the translocation t(15; 17) can be unequivocally identified even if the spread and the morphology of the chromosomes are poor. The sensitivity of CISS hybridization is compared with the sensitivity of conventional G-banded karyotypes.
在本研究中，我们证明了染色体原位抑制（CISS）杂交技术在检测急性早幼粒细胞白血病患者中期分裂相中t(15; 17)易位的可行性。使用从人15号和17号染色体分选得到的DNA文库，即使染色体的分散状态和形态不佳，也能明确鉴定出t(15; 17)易位。将CISS杂交技术的敏感性与传统G显带核型分析的敏感性进行了比较。
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