Rojas-Rousse D, Bigot Y, Periquet G
Institut de Biocénotique Expérimentale des Agrosystèmes, Université Francois Rabelais, Tours, France.
Mol Biol Evol. 1993 Mar;10(2):383-96. doi: 10.1093/oxfordjournals.molbev.a040010.
In the two parasitoid wasps, Diadromus collaris and Eupelmus orientalis, the satellite DNAs were each found to consist wholly or largely of a single family (5%-7% of the genome). Several clones of each family were obtained and sequenced. The repeat unit in each species is characterized by both the repetition of a basic motif and the presence of an inserted sequence. Sequence comparisons with satellite DNA from D. pulchellus and E. vuilleti provide plausible scenarios for the evolution of the satellite DNA in each genus. Palindromes and A-rich tracts in each consensus sequence suggest the formation, in vivo, of hairpin structures and bend centers that may play a role in heterochromatin condensation in insects. The insertions in the repeat units of each species also contain these structural features, suggesting that maintenance of these insertions requires constraints similar to those pertaining to the rest of the satellite-DNA unit.
在两种寄生蜂——颈双缘姬蜂（Diadromus collaris）和东方真釉小蜂（Eupelmus orientalis）中，发现卫星DNA均全部或大部分由单一家族组成（占基因组的5%-7%）。每个家族获得了几个克隆并进行了测序。每个物种中的重复单元的特征是基本基序的重复以及插入序列的存在。与秀丽双缘姬蜂（D. pulchellus）和维氏真釉小蜂（E. vuilleti）的卫星DNA进行序列比较，为每个属中卫星DNA的进化提供了合理的推测。每个共有序列中的回文序列和富含A的区域表明，在体内形成了可能在昆虫异染色质浓缩中起作用的发夹结构和弯曲中心。每个物种重复单元中的插入序列也包含这些结构特征，这表明维持这些插入序列需要与卫星DNA单元其他部分类似的限制条件。
