Hernández-Caselles T, Villalaín J, Gómez-Fernández J C
Departamento de Bioquímica y Biología Molecular A, Facultad de Veterinaria, Universidad de Murcia, Spain.
Mol Cell Biochem. 1993 Mar 24;120(2):119-26. doi: 10.1007/BF00926084.
Lipid composition and specially their electrostatic properties, were found to greatly influence the stability of liposomes in human blood serum. The amount and type of serum proteins bound to the liposomes were also clearly influenced by lipid composition and charge of liposomes. A good correlation was found between the amount of serum proteins adsorbed to a given type of liposome and its instability as measured by the release of an encapsulated fluorescent probe. Liposomes that bind the highest amount of protein were the least stable, except for the case of liposomes containing gangliosides, which were fairly stable even at a high amount of bound protein. Liposomes with neutral charge containing phosphatidylcholine were the most stable and bound the lowest amount of protein. Liposomes with positive charge behaved similarly to those with neutral charge. However, the stability of negatively charged liposomes was very dependent on their composition. Those liposomes containing only one class of negatively charged phospholipids bound a great amount of protein and were very unstable. However, those liposomes containing also phosphatidylcholine bound less protein and were more stable. The examination of the electrophoresis patterns of serum proteins bound to the different types of liposomes indicated the presence of specific proteins which correlated with liposome instability.
脂质组成，特别是其静电性质，被发现对脂质体在人血清中的稳定性有很大影响。结合到脂质体上的血清蛋白的数量和类型也明显受到脂质体的组成和电荷的影响。在吸附到给定类型脂质体上的血清蛋白数量与其通过包封的荧光探针释放所测量的不稳定性之间发现了良好的相关性。结合蛋白量最高的脂质体最不稳定，但含有神经节苷脂的脂质体除外，即使在结合蛋白量很高时它们也相当稳定。含有磷脂酰胆碱的中性电荷脂质体最稳定，结合的蛋白量最低。带正电荷的脂质体表现与带中性电荷的脂质体相似。然而，带负电荷脂质体的稳定性非常依赖于它们的组成。那些仅含有一类带负电荷磷脂的脂质体结合大量蛋白且非常不稳定。然而，那些也含有磷脂酰胆碱的脂质体结合的蛋白较少且更稳定。对结合到不同类型脂质体上的血清蛋白的电泳图谱的检查表明存在与脂质体不稳定性相关的特定蛋白。
