Shchelkunova T A, Smirnov A N, Faktor V M, Brodskiĭ V Ia, Rozen V B
Ontogenez. 1993 Mar-Apr;24(2):70-5.
Radioligand and immunohistochemical analysis was used for studying the expression of an unusual estrogen-binding protein (UEBP) in the regenerated liver of male rats poisoned with CCl4. As a result of CCl4 poisoning, most hepatocytes intensely producing UEBP and located in the central part of the liver lobules are destroyed (40 to 90% of the liver parenchymal cells). Dead cells are substituted by new hepatocytes formed due to proliferation of periportal hepatocytes in which UEBP is expressed at a low level. In regenerated liver, however, a high level of UEBP expression characteristic of the control animals is restored, as well as a gradient mode of distribution of UEBP-containing cells with the maximum UEBP concentration in hepatocytes surrounding the central vein. The results obtained with animals with either intact or removed testes were similar, except that the dynamics of liver regeneration was slower in the castrated rats. These data suggest the existence in all hepatocytes of the complete androgen-related program for UEBP expression. This program is inherited by daughter cells in the absence of an inducing hormone and is similar in all cells, independently of the extent of its phenotypic expression in hepatocytes with different localization within the liver lobule.
采用放射性配体和免疫组织化学分析方法，研究了四氯化碳中毒雄性大鼠再生肝中一种异常雌激素结合蛋白（UEBP）的表达情况。四氯化碳中毒导致大部分强烈产生UEBP且位于肝小叶中央部分的肝细胞被破坏（占肝实质细胞的40%至90%）。死亡细胞被由门静脉周围肝细胞增殖形成的新肝细胞替代，这些门静脉周围肝细胞中UEBP表达水平较低。然而，在再生肝中，恢复了对照动物特有的高水平UEBP表达，以及含UEBP细胞的梯度分布模式，中央静脉周围肝细胞中的UEBP浓度最高。完整睾丸或摘除睾丸的动物所获得的结果相似，只是去势大鼠的肝再生动力学较慢。这些数据表明，所有肝细胞中都存在完整的与雄激素相关的UEBP表达程序。该程序在没有诱导激素的情况下由子细胞继承，并且在所有细胞中相似，与它在肝小叶内不同位置的肝细胞中的表型表达程度无关。
