[萤火虫荧光素酶的生物分析用途（综述）]
[The bioanalytical uses of the luciferase from fireflies (a review)].
作者信息
Ugarova N N
出版信息
Prikl Biokhim Mikrobiol. 1993 Mar-Apr;29(2):180-92.
PMID:8488159
Abstract
Main principles of the bioluminescent microassay with the use of firefly luciferase are considered. Literature data and own experimental results of the author on application of luciferase in microbiology, clinical biochemistry, and express assays for antibiotic sensitivity and resistance to biocorrosion are generalized. New approaches to the use of luciferase for the detection of enzyme labels in EIA and for the nonradioactive detection of DNA probes are discussed.
摘要
本文探讨了利用萤火虫荧光素酶进行生物发光微量分析的主要原理。概括了文献数据以及作者自身关于荧光素酶在微生物学、临床生物化学、抗生素敏感性快速检测和生物腐蚀抗性方面应用的实验结果。讨论了利用荧光素酶检测酶免疫分析中的酶标记物以及非放射性检测DNA探针的新方法。
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