Coyle P, Feng X
Department of Anatomy and Cell Biology, University of Michigan, Ann Arbor 48109-0616.
Stroke. 1993 May;24(5):705-9; discussion 710. doi: 10.1161/01.str.24.5.705.
Our purpose was to characterize the surface area of the infarct and the surface area at risk of infarction as defined spatially by arterial anastomoses to determine whether position, size, or shape of the infarct and the area at risk were related in stroke-prone rats or hybrid rats.
Stroke-prone rats (n = 18; mean +/- SEM blood pressure, 182 +/- 8 mm Hg) and hybrid rats (n = 18; mean +/- SEM blood pressure, 147 +/- 6 mm Hg; p < 0.05) were anesthetized and the left middle cerebral artery was occluded with a ligature. The rats were killed 7 days later, arterial anastomoses were made visible with latex, the brains were fixed in formalin, and film recorded the infarct and anastomoses. Anastomoses and infarcts were digitized for measurements of risk area, luminal width, and infarct area.
Mean risk area was similar in size, length, width, and variability in stroke-prone rats (area, 85 +/- 5 mm2) and hybrid rats (area, 84 +/- 7 mm2; p > 0.05), whereas mean infarct area was larger, longer, wider, and less variable in stroke-prone rats (area, 53 +/- 6 mm2) than in hybrid rats (area, 15 +/- 11 mm2; p < 0.05). Infarct length was appreciably greater than infarct width in both groups, indicating that infarct shape was not amorphous. Spatial overlap maps indicated that the infarct area common to all stroke-prone rats was positioned centrally in the risk area and was surrounded by a variable infarct area, which indicated that the likelihood of infarction increased with distance from the anastomoses. Shape factors for both risk area and infarct area were significantly different within each rat group, which indicated that infarct shape did not uniformly parallel the anastomotic sites that determined risk area shape (p < 0.05). Risk area anastomoses and border zone width were linearly correlated in size and both were significantly wider in hybrid rats than in stroke-prone rats (p < 0.05), which suggests that the narrower border zone tissue was perfused by narrower anastomoses.
We conclude that the position of the infarct within the risk area relates to luminal widths of conterminous anastomoses that define the risk area, but not to the size or shape of the area at risk of infarction defined spatially by the anastomoses.
我们的目的是描绘梗死灶的表面积以及根据动脉吻合在空间上定义的梗死风险区域的表面积，以确定梗死灶的位置、大小或形状以及风险区域在易中风大鼠或杂种大鼠中是否相关。
将易中风大鼠（n = 18；平均±标准误血压，182±8 mmHg）和杂种大鼠（n = 18；平均±标准误血压，147±6 mmHg；p < 0.05）麻醉，用结扎线闭塞左大脑中动脉。7天后处死大鼠，用乳胶使动脉吻合可见，将大脑固定在福尔马林中，并用胶片记录梗死灶和吻合情况。将吻合和梗死灶数字化以测量风险区域、管腔宽度和梗死面积。
易中风大鼠（面积，85±5 mm²）和杂种大鼠（面积，84±7 mm²；p > 0.05）的平均风险区域在大小、长度、宽度和变异性方面相似，而易中风大鼠（面积，53±6 mm²）的平均梗死面积比杂种大鼠（面积，15±11 mm²；p < 0.05）更大、更长、更宽且变异性更小。两组的梗死长度均明显大于梗死宽度，表明梗死灶形状并非无定形。空间重叠图显示，所有易中风大鼠共有的梗死区域位于风险区域的中央，并被一个可变的梗死区域包围，这表明梗死的可能性随与吻合处距离的增加而增加。每个大鼠组内风险区域和梗死区域的形状因子均有显著差异，这表明梗死灶形状并非与决定风险区域形状的吻合部位均匀平行（p < 0.05）。风险区域吻合和边缘带宽度在大小上呈线性相关，且杂种大鼠两者均明显宽于易中风大鼠（p < 0.05），这表明较窄的边缘带组织由较窄的吻合供血。
我们得出结论，梗死灶在风险区域内的位置与界定风险区域的相邻吻合处的管腔宽度有关，但与由吻合在空间上定义的梗死风险区域的大小或形状无关。
