Gratton J A, Sauter A, Rudin M, Lees K R, McColl J, Reid J L, Dominiczak A F, Macrae I M
Wellcome Surgical Institute, University of Glasgow, Scotland, UK.
Stroke. 1998 Mar;29(3):690-4. doi: 10.1161/01.str.29.3.690.
Susceptibility to cerebral infarction was compared in stroke-prone spontaneously hypertensive (SHRSP), normotensive Wistar-Kyoto (WKY) rats, and F1 hybrids derived from a SHRSP/WKY cross.
The proximal left middle cerebral artery (MCA) was occluded under anesthesia and infarct volume assessed 24 hours later by magnetic resonance imaging and confirmed 5 days later by quantitative histopathology. Total hemispheric infarct volume was expressed as a percentage of the total brain volume.
Infarct volumes measured by MRI in adult SHRSP (19.5 +/- 2.0%) and F1 hybrid rats (19.4 +/- 1.9%) were significantly greater than in WKY (11.1 +/- 2.4; CI [6.07, 10.76]) and (5.93, 10.52), respectively, P<.001). Sensitivity to an ischemic insult was unrelated to blood pressure: although systolic blood pressures differed between young versus adult male SHRSP and between female versus male SHRSP and F1 hybrids, infarct volumes were equal. A close correlation was found between infarct volumes measured by MRI and histology (r=.92, P<.0001).
Outcome to MCA occlusion (MCAO) measured with MRI provides a reproducible and nonterminal quantitative phenotypic marker of stroke susceptibility in the SHRSP. This is the first study to employ MCAO with MRI to quantify stroke susceptibility in F1 hybrid rats and indicates a dominant mode of inheritance for this phenotype.
比较易患中风的自发性高血压大鼠（SHRSP）、血压正常的Wistar-Kyoto（WKY）大鼠以及SHRSP/WKY杂交产生的F1代杂种大鼠发生脑梗死的易感性。
在麻醉状态下阻断左侧大脑中动脉近端，24小时后通过磁共振成像评估梗死体积，并在5天后通过定量组织病理学加以确认。全脑半球梗死体积以占全脑体积的百分比表示。
成年SHRSP大鼠（19.5±2.0%）和F1代杂种大鼠（19.4±1.9%）通过MRI测量的梗死体积显著大于WKY大鼠（分别为11.1±2.4；CI[6.07,10.76]）和（5.93,10.52），P<0.001）。对缺血性损伤的敏感性与血压无关：尽管年轻与成年雄性SHRSP大鼠之间以及雌性与雄性SHRSP和F1代杂种大鼠之间的收缩压有所不同，但梗死体积相同。通过MRI和组织学测量的梗死体积之间存在密切相关性（r=0.92，P<0.0001）。
用MRI测量大脑中动脉闭塞（MCAO）的结果可提供一种可重复且非终末性的定量表型标记，用于评估SHRSP大鼠中风易感性。这是第一项采用MCAO联合MRI来量化F1代杂种大鼠中风易感性的研究，表明该表型具有显性遗传模式。
