Hayakawa S, Chishima F, Sakata H, Fujii K, Ohtani K, Kurashina K, Hayakawa J, Suzuki K, Nakabayashi H, Esumi M
Department of Obstetrics and Gynecology, Nihon University School of Medicine, Tokyo, Japan.
Transfusion. 1993 May;33(5):413-7. doi: 10.1046/j.1537-2995.1993.33593255603.x.
A woman with recurrent Paget's disease of the vulva developed acute graft-versus-host disease (GVHD) 12 days after radical surgery and massive blood transfusion. Molecular diagnosis of lymphocyte chimerism in the peripheral blood was made by polymerase chain reaction (PCR) directed against a Y chromosome-specific sex-determining region Y (SRY) gene. PCR with skin biopsy after onset of GVHD also revealed infiltration of SRY-positive donor lymphocytes. The diagnosis was confirmed by HLA-DNA typing with PCR-sequence-specific oligonucleotide that revealed the presence of complex HLA-DR chimerism in the peripheral lymphocytes collected after onset of GVHD. The use of SRY-directed PCR is a rapid technique for the early diagnosis of acute posttransfusion GVHD in female patients.
一名复发性外阴佩吉特病女性在根治性手术和大量输血后12天发生急性移植物抗宿主病（GVHD）。通过针对Y染色体特异性性别决定区Y（SRY）基因的聚合酶链反应（PCR）对外周血淋巴细胞嵌合体进行分子诊断。GVHD发病后皮肤活检的PCR也显示SRY阳性供体淋巴细胞浸润。通过PCR序列特异性寡核苷酸进行HLA - DNA分型确诊，结果显示GVHD发病后采集的外周淋巴细胞中存在复杂的HLA - DR嵌合体。使用针对SRY的PCR是早期诊断女性患者急性输血后GVHD的快速技术。
