Hayakawa S, Satoh K
Dept. Obstetrics and Gynaecology, Nihon University School of Medicine.
Nihon Rinsho. 1997 Sep;55(9):2272-6.
Graft-versus-host disease (GVHD) is one of the most serious complications of blood transfusion. Thus, it is important to make a correct diagnosis of post-transfusion GVHD as soon as possible in its onset. Presence of Y-chromosome positive donor lymphocytes in the peripheral blood of female GVHD patients is one of the direct evidences of lymphocyte chimerism, as well as shown by HLA chimerism. We have reported a rapid diagnosis technique of post-transfusion GVHD by polymerase chain reactions directed against Y-chromosome specific SRY gene. (Hayakawa S, et al: Transfusion. 1993, 33, 413-417). We can detect single male cell in 10,000 female cells by SRY directed nested PCR. Though this method is applicable only for the female GVHD cases involved with Y-chromosome positive male lymphocytes, it is possible to detect not only chimeric peripheral lymphocytes but also skin infiltrating donor lymphocytes.
移植物抗宿主病（GVHD）是输血最严重的并发症之一。因此，在输血后GVHD发病时尽快做出正确诊断非常重要。女性GVHD患者外周血中存在Y染色体阳性供体淋巴细胞是淋巴细胞嵌合的直接证据之一，HLA嵌合也可显示这一点。我们已经报道了一种通过针对Y染色体特异性SRY基因的聚合酶链反应快速诊断输血后GVHD的技术。（Hayakawa S等人：《输血》。1993年，33卷，413 - 417页）。通过SRY定向巢式PCR，我们可以在10000个女性细胞中检测到单个男性细胞。虽然这种方法仅适用于涉及Y染色体阳性男性淋巴细胞的女性GVHD病例，但不仅可以检测嵌合外周淋巴细胞，还可以检测皮肤浸润的供体淋巴细胞。
