Littlechild J A, Watson H C
Department of Chemistry, University of Exeter, UK.
Trends Biochem Sci. 1993 Feb;18(2):36-9. doi: 10.1016/0968-0004(93)90048-r.
The structures of three type I fructose-1,6-bisphosphate aldolases have been determined and the common residues surrounding the Schiff base-forming Lys residue located. Armed with this information, it is now possible to propose a mechanism for this ubiquitous enzyme which is consistent with the recorded biochemical data. An interesting, but by no means mandatory, feature of the reaction mechanism is that catalysis can proceed without exchange with the solvent.
已确定了三种I型果糖-1,6-二磷酸醛缩酶的结构，并定位了围绕形成席夫碱的赖氨酸残基的常见残基。有了这些信息，现在就有可能提出一种与已记录的生化数据一致的、适用于这种普遍存在的酶的机制。反应机制的一个有趣但绝非必要的特征是，催化作用可以在不与溶剂交换的情况下进行。
