Aggarwal S K, Gemma N W, Kinter M, Nicholson J, Shipe J R, Herold D A
Department of Pathology, University of Virginia Health Sciences Center, Charlottesville 22908.
Anal Biochem. 1993 Apr;210(1):113-8. doi: 10.1006/abio.1993.1159.
Isotope dilution gas chromatography-mass spectrometry (GC-MS) and electrothermal atomic absorption spectrometry (EAAS) are compared for platinum (Pt) determination in urine, plasma ultrafiltrate, and plasma samples from a patient undergoing cisplatin therapy. The isotope dilution GC-MS method is based on the use of lithium bis(trifluoroethyl)dithiocarbamate as a chelating agent and enriched 192Pt as an internal standard. Pt isotope ratios were measured using a Finnigan MAT 8230 organic mass spectrometer, and Pt concentrations were calculated from different sets of isotope ratios in the molecular ion of the Pt-chelate. In the EAAS method, Pt concentrations were determined using three different approaches. These were: (i) calibration curve based on aqueous standards containing Pt in 10% HCl, (ii) standard addition, and (iii) matrix digestion followed by standard addition. Good agreement was obtained for Pt concentrations determined by GC-MS and EAAS in urine samples while there were significant differences in Pt concentrations of ultrafiltrate and whole plasma samples by the two methods. Discussion of possible reasons for these differences emphasizes the need for future critical evaluation of these methods.
对顺铂治疗患者的尿液、血浆超滤液和血浆样本中的铂（Pt）测定，比较了同位素稀释气相色谱 - 质谱联用（GC - MS）法和电热原子吸收光谱法（EAAS）。同位素稀释GC - MS法基于使用双（三氟乙基）二硫代氨基甲酸锂作为螯合剂，并使用富集的¹⁹²Pt作为内标。使用菲尼根MAT 8230有机质谱仪测量Pt同位素比率，并根据Pt螯合物分子离子中不同组的同位素比率计算Pt浓度。在EAAS法中，使用三种不同方法测定Pt浓度。这些方法是：（i）基于含10% HCl中Pt的水溶液标准品的校准曲线，（ii）标准加入法，以及（iii）基质消解后再采用标准加入法。尿液样本中通过GC - MS和EAAS测定的Pt浓度结果吻合良好，而两种方法测定超滤液和全血样本的Pt浓度存在显著差异。对这些差异可能原因的讨论强调了未来对这些方法进行严格评估的必要性。
